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PromSil HPLC columns are made of spherical silica particles manufactured of the highest purity (> 99.999%) in the market. The highest quality silica, an innovative surface nano-modification and unique bonding processes result in exceptional C18 phases with outstanding properties.  Lot-to-lot reproducibility is ensured by a stable bonding/end-capping process that is extremely rugged. The uniform, spherical particles have a nominal surface area of 308 m2/g with a controlled pore size of 100 Å or 200 m2/g with a pore size of 150 Å. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, lifetime and column-to-column reproducibility. To meet different application needs within the corresponding pH requirements, we have the following offers available in our reverse phase product line.

PromSil PXB

PromSil PSB-C18

PromSil AQ-C18

PromSil PXB series, PXB-C18 and PXB-C8, are bonded to the maximum density to have the highest hydrophobicity (the lowest polarity), the lowest interaction of analytes with silanols, high stability at high pH and the broadest pH range.

PromSil PSB-C18 columns are the most polar among C18 columns and are good alternatives to Zorbax SB-C18. They are extremely stable at low pH and may also be used at mid-pH. They are also friendly towards basic compounds at low- to mid-pH and are compatible with 100% aqueous mobile phase.

PromSil AQ-C18 columns are slightly polar than PXB and have more separation power for polar compounds. Due to special silica treatment, they are true C18 columns that are compatible with 100% aqueous mobile phase. Like PXB columns, they are very friendly towards basic analytes. They are good alternatives to Waters Atlantis. AQ-C18 has the broadest application range.

Comparison of PromSil -C18 Phases

Property

PXB-C18

AQ-C18

PSB-C18

Surface area (m2/g)

380

380

200

C%

22

18

11

End-cap

Double

Single

Polar

Polarity increase

 

>>>>>  

 

pH stability

1.5-10.0

1.5-9.0

0.8-7.0

100% water compatible

No

Yes

Yes

Peak shape of bases

Excellent

Excellent

Excellent

Alternative to

SunFire C18
Luna C18
Discovery C18

Symmetry C18
Atlantis dC18
Inertsil ODS-3

Zorbax SB C18
Zorbax SB-AQ
Waters Atlantis

Selectivity Comparison of the PromSil Phases

a. PXB-C8
60% MeOH
α(TOL/CNB) = 1.61

b. PXB-C18
65% MeOH
α(TOL/CNB )= 1.81

c. AQ-C18
65% MeOH
α(TOL/CNB) = 1.55

d. PSB-C18
65% MeOH
α(TOL/CNB)=1.65

Sample:  Uracil, phenol, chloronitrobenzene (CNB), Toluene (TOL); column2.1 x 50mm, 5µm; solvent: MeOH/water; flow rate: 0.2 mL/min; injection volume: 1 µL;  temperature: 30 º C; detection: UV 254nm

Cross-Reference for PromSil Series Columns

PromSil  Column

PXB-C18

PXB-C8

PXB(L)-C18

PXB(L)-C8

PSB-C18

PSB-C8

AQ-C18

HILIC

Agilent

 

 

 

 

 

 

 

 

SB-C18

 

 

 

 

R

 

 

 

SB-C8

 

 

 

 

 

R

 

 

XDB-C18

 

 

R

 

 

 

 

 

XDB-C8

 

 

 

R

 

 

 

 

SB-AQ

 

 

 

 

 

 

R

 

Waters

 

 

 

 

 

 

 

 

SunFire C18

R

 

 

 

 

 

 

 

SunFire C8

 

R

 

 

 

 

 

 

Atlantis-C18 (AQ)

 

 

 

 

 

 

E

 

Atlantis-HILIC

 

 

 

 

 

 

 

R

Phenomenex

 

 

 

 

 

 

 

 

Luna-C18

E

 

 

 

 

 

 

 

Luna-C8

 

E

 

 

 

 

 

 

Supelco

 

 

 

 

 

 

 

 

Discovery-C18

 

 

E

 

 

 

 

 

Discovery-C8

 

 

 

E

 

 

 

 

Asentics-C18

E

 

 

 

 

 

 

 

Asentics-C8

 

E

 

 

 

 

 

 

GL Sciences

 

 

 

 

 

 

 

 

Inertsil ODS-3

 

 

 

 

 

R

 

 

E: similar selectivity and superior performance

R: a better replacement