LC column, HPLC column, HILIC column, mixed mode column, halo column, gpc column, zorbax column, luna column

Mixed Mode Column   HILIC Column    Reverse Phase Column    Rapid Analysis Column   GPC Column   Column for Life Science  Mini Column  Instrument   Controller    Order    Home    

HILIC (Hydrophilic Interaction LIquid Chromatography) is a special case of normal phase chromatography. It is used primarily for separation of very polar compounds. The typical mobile phase for HILIC chromatography includes acetonitrile with a small water content. It is commonly believed that in HILIC, the mobile phase forms a water rich layer on the surface of the polar stationary phase creating a liquid/liquid extraction system. On HILIC mode, polar compounds have longer retention time than non-polar compounds. It avoids using ion-pair reagents or high water content for separation of  very polar compounds and provides better peak shapes for basic compounds. The high concentration of organic solvent in the mobile phase pushes up the sensitivity of LC-MSD analysis.

There are several types of HILIC columns available. Due to involvement of other separation mechanisms, the elution profile for each HILIC column can be different. PromoChrom offers 3 types of HILIC columns to meet different needs.

Venusil HILIC: Unique HILIC column for small molecules. Venusil HILIC column is based on bare silica. The surface of the silica is bonded with urea functional group to stabilize the stationary phase and to minimize interaction of analytes with the silica. Ideal for small polar molecules.   More Information

Obelisc N: The most versatile HILIC column with widest application range. In addition to normal phase and HILIC modes, Obelisc N also provides ion exchange mechanism for negatively charged ions. Due to its multiple separation mechanism, the column may be used to separate almost any compounds. More Information

PolyHYDROXYETHYL A - An excellent HILIC column for biological molecules (From PlolyLC). The column is excellent for separation of peptides, nucleic acids, carbohydrates, some proteins. Its great polarity means less organic solvent is needed to get retention compared to other HILIC columns. In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode. It comes with different pore sizes (60 - 300 Å) for different applications.