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Hyper Fast and Super Rugged HPLC Columns Based on Fused Core Particle Technology

Fused-Core particle technology was developed by Jack Kirkland to produce HPLC columns that provide faster separations and higher sample throughput without sacrificing column ruggedness and reliability. As the name implies, Fused-Core particles are manufactured by “fusing” a porous silica layer onto a solid silica particle.

The shorter diffusion path of HALO particles reduces axial dispersion of solutes and minimizes peak broadening. A Halo particle has only a 0.5 µm diffusion path compared to the approximately1.5 µm diffusion path of a 3 µm totally porous particle. Because of the shorter diffusion path, the performance advantages of HALO become even more apparent when separating larger solute molecules and operating at faster mobile phase flow rates.

HALO columns deliver over 50% more separating power (theoretical plates) than a column of the same length packed with 3.5 µm particles and more than twice the plates of a column packed with 5 µm particles. It means the separation power of a 50 mm HALO column is equivalent to a 200 mm column packed with 5 µm particles.

And, because of Fused-Core particle technology, HALO columns maintain their resolving power at high flow rates. This means that shorter columns and higher flow rates can be used to achieve remarkably fast high resolution separations.

Peak tailing due to trace metals or silanol groups is essentially non-existent on HALO stationary phases. The examples here show the excellent peak shape that can be achieved for either bases or acids when using a HALO column. The conditions used for the bases were chosen to encourage any potential silanol interference. Note the excellent peak shape for amitriptyline under these conditions.

Specifications: Stationary Phase Support Ultra-pure, “Type B” silica 1.7 µm solid core particle with a 0.5 µm porous silica layer fused to the surface 150 m2/gram surface area 90 Å pore size Bonded Phase Monomeric bonding chemistry Densely bonded phase Maximized endcapping C18: Octadecyldimethylsilane, 3.5 µmoles/m2 C8: Octyldimethylsilane, 3.7 µmoles/m2 pH range: 2 to 9 Maximum Pressure: 9,000 psi, 600 bar

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